Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Identification of Novel Laminin- and Fibronectin-binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2

doi: 10.3389/fcimb.2015.00082

Figure Lengend Snippet: Determine the binding of the recombinant proteins to LN and FN by Far-Western blot . Coomassie G-250-stained gel (A) , Western blot analysis (B) , and Far-Western blot analysis (C,D) of the SS2 recombinant proteins. Recombinant proteins were separated by 12% SDS-PAGE, then transferred to PVDF membrane and incubated with human LN or LN. Bound LN or FN was detected with goat anti-rabbit IgG antibody. The Western blot (B) was probed with his tag monoclonal antibody (Boster).

Article Snippet: His tag monoclonal antibody (Boster; 1:1000 dilution) were added to every plate and wells were then incubated at 37°C for 30 min. After washing, the cultured cells were incubated with goat anti-mouse IgG-FITC (Boster; 1:2000 dilution) at 37°C for 30 min. After a finial wash, the samples were examined using a fluorescence microscope (ZEISS, Germany).

Techniques: Binding Assay, Recombinant, Far Western Blot, Staining, Western Blot, SDS Page, Membrane, Incubation

Journal: Nutrients

Article Title: The Effect of Broccoli Sprout Extract on Seasonal Grass Pollen-Induced Allergic Rhinitis

doi: 10.3390/nu13041337

Figure Lengend Snippet: Breakdown of GST polymorphisms for each group. GST = glutathione S-transferase. Gene families divided into M1 null, M1, 2, 3 or 4 vs. T1 null, T1, 2 vs. P1G/G, A/G or A/A.

Article Snippet: GSTM1 and GSTT1 copy number was determined using the SNP genotyping assays Hs02575461_cn and Hs00010004_cn with human RnaseP as TaqMan copy number reference and genotyping master mix from Applied Biosystems (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Activity Assay